Conventional and molecular identification of Brucella isolates from livestock in Turkey

The main object of the study is to demonstrate that Bruce-ladder multiplex PCR, which capable identify all Brucella species including vaccinal strains, a safe and practical method alternative bacteriological culture methods. Brucellosis zoonosis great socio-economic importance endemic in Turkey. In three-year period from 2009 2011, total 281 spp. isolates cattle, sheep goats were identified characterized by both conventional biotyping procedures PCR based called Bruce-ladder. Species identification phenotypic testing was concordant for tested. results showed preferred host pattern B.abortus isolates. terms identity, it found 94% isolations derived cattle exclusively biotype 3. B.melitensis 3 1 prevelant biotypes goats. Two as Rev.1 vaccine strain. Surprisingly, one goat S19 Other interesting observations isolation penicilin-sensitive non strains well an atypical strain on basis growth inhibition presence basic fuchsin dye. At end this study, concluded monitoring them are indispensable epidemiological tool brucellosis control